bulk-rnaseq

SAFE max severity SAFESkillSpector Caution 48 registry K-Dense-AI last scanned 1 scan

No security findings. Scanned 1 time, most recently 2026-10-21.

Second scanner: NVIDIA SkillSpector

Independent static run, no language model, over the same files as the Cisco scan above. How the two scanners compare across the whole corpus: Two scanners, one corpus.

What this skill does

Prepares bulk RNA-seq FASTQ, Salmon, STAR or featureCounts output for gene-level differential expression. Covers nf-core/rnaseq and standalone quantification, biological replication, strandedness, reference provenance, validated count assembly and a PyDESeq2 handoff. Use for FASTQ-to-counts analysis, nf-core/rnaseq configuration, STAR/Salmon quantification, or building a counts matrix for DESeq2. For single-cell data use scanpy; for statistical fitting alone use pydeseq2.

Package
8 files, 71 KB
SKILL.md
2040 words, 12+ sections
Code
Python (3)
License
MIT
Compatibility
Requires Python 3.11+ with pandas and numpy; Salmon import also needs pytximport. Read processing needs Nextflow with containers or standalone bioinformatics tools. Network access is needed for installation and reference downloads.

SKILL.md covers: Bulk RNA-seq · Overview · When to Use This Skill · The Pipeline at a Glance · Two Upstream Paths — Pick One · Setup · This skill's glue (bridge + handoffs) — Python · Downstream skills install their own deps: · pydeseq2 skill -> uv pip install pydeseq2 · pathway-enrichment skill -> uv pip install gseapy gprofiler-official · Path A (nf-core): only Nextflow + a container engine are needed — see the `nextflow` skill. · Path B (standalone tools): install via bioconda. Pin versions for reproducibility.

Findings

No findings. SAFE

What is in the package

FileKindSize
SKILL.mddoc16 KB
references/counts-and-handoff.mddoc8.3 KB
references/design-and-qc.mddoc6.2 KB
references/upstream-manual.mddoc7.0 KB
references/upstream-nfcore.mddoc7.3 KB
scripts/_tabular.pyscript975 B
scripts/build_counts_matrix.pyscript14 KB
scripts/validate_samplesheet.pyscript10 KB

External hosts referenced

github.com nf-co.re pytximport.complextissue.com arxiv.org bioconductor.org pydeseq2.readthedocs.io combine-lab.github.io docs.seqera.io doi.org export.arxiv.org multiqc.info subread.sourceforge.net www.bioinformatics.babraham.ac.uk

Hostnames that appear in the skill's files. Referencing a host is not a finding; compare against the findings above and the skill's stated purpose.

Package contents recorded 2026-10-22 at commit 593377bb0487. The scan above ran against the version current at scan time; the two can differ if the skill has since been updated.

What we checked

What we did not check

Sources